[1]李辽源 吴杰英 刘绍远 杨明 罗子寰 张凌霄 高新 孟占鳌.膀胱尿路上皮癌组织中DACH2 的表达及临床意义[J].中华腔镜泌尿外科杂志(电子版),2016,10(06):415-419.[doi:10.3877/cma.j.issn.1674-3253.2016.06.012 ]
 Li Liaoyuan,Wu Jieying,Liu Shaoyuan,et al.Expression of dachshund homolog 2 gene in human bladder urothelial cancer and its clinical significance[J].,2016,10(06):415-419.[doi:10.3877/cma.j.issn.1674-3253.2016.06.012 ]
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膀胱尿路上皮癌组织中DACH2 的表达及临床意义()
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中华腔镜泌尿外科杂志(电子版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
10
期数:
2016年06期
页码:
415-419
栏目:
临床研究
出版日期:
2016-12-31

文章信息/Info

Title:
Expression of dachshund homolog 2 gene in human bladder urothelial cancer and its clinical significance
作者:
李辽源 1吴杰英 1刘绍远 2杨明 2罗子寰 1张凌霄 1高新 1孟占鳌 3
510630广州,中山大学附属第三医院泌尿外科 1,放射科 3;528000广东,佛山市第一人民医院泌尿外科 2
Author(s):
Li Liaoyuan1 Wu Jieying1 Liu Shaoyuan2 Yang Ming2 Luo Zihuan1 Zhang Lingxiao1 Gao Xin1 Meng Zhan'ao3.
Department of Urology, 3Department of Radiology, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510630, China ; 2Department of Urology, the First People’s Hospital of Foshan City, Guangdong 528000, China
关键词:
尿路上皮肿瘤膀胱 DACH2分期分级
DOI:
10.3877/cma.j.issn.1674-3253.2016.06.012
摘要:
目的探讨 DACH2基因在膀胱尿路上皮癌组织中的表达情况,并分析其与膀胱癌临床病理特征及复发的关系。方法应用免疫组化技术( SP法)和实时定量聚合酶链反应( qRT-PCR)法检测 47例膀胱尿路上皮癌组织及 11例癌旁正常膀胱黏膜组织中 DACH2的表达,并结合临床病理资料进行分析。结果免疫组化结果显示 DACH2蛋白在肿瘤组表达高于正常膀胱黏膜组( P< 0.05);随分级、分期增高, DACH2蛋白组间表达差异有统计学意义( P< 0.05)。qRT-PCR显示 DACH2的 mRNA表达在肿瘤组( 2-△△Ct 18.25)高于正常膀胱黏膜( 2-△△Ct 1.00)(P< 0.05);复发组( 2-△△Ct 19.70)高于原发组( 2-△△Ct 7.57)(P<0.05);随膀胱癌的分级、分期增高, DACH2的 mRNA组间表达差异有统计学意义( < 0.05)。结论 DACH2高表达与膀胱尿路上皮癌的恶性侵袭性明显相关,检测 DACH2有助于膀胱癌的分期和分级。

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:第 1批中国博士后科学基金特别资助项目( 200801264);第 42批中国博士后科学基金项目(20070420795); 2008年广东省医学科研基金项目( B2008164)
通讯作者:孟占鳌,Email:mengzhanao0806@163.com
本文编辑:阮星星 李文标) 李辽源,吴杰英,刘绍远,等.膀胱尿路上皮癌组织中 DACH2的表达及临床意义[J/CD].中华腔镜泌尿外科杂志:电子版, 2016, 10(6
更新日期/Last Update: 2016-12-30