[1]徐汉新,陈华忠,吴兆春,等.抗增殖因子通过下调紧密连接蛋白 Zo-1、 Occludin的表达促进间质性膀胱炎的发生[J].中华腔镜泌尿外科杂志(电子版),2015,(02):131-135.[doi:10.3877/cma.j.issn.1674-3253.2015.02.013 ]
 Xu Hanxin,Chen Huazhong,Wu Zhaochun,et al.APF promotes the occurrence of interstitial cystitis through down regulate expression of Zo-1 and occludin[J].,2015,(02):131-135.[doi:10.3877/cma.j.issn.1674-3253.2015.02.013 ]
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抗增殖因子通过下调紧密连接蛋白 Zo-1、 Occludin的表达促进间质性膀胱炎的发生()
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中华腔镜泌尿外科杂志(电子版)[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
131-135
栏目:
·短篇论著·
出版日期:
2015-08-31

文章信息/Info

Title:
APF promotes the occurrence of interstitial cystitis through down regulate expression of Zo-1 and occludin
作者:
徐汉新1陈华忠 1吴兆春1刘文彬 1杨邦彦1黄海2
523660 广东东莞,东莞市清溪医院泌尿外科1;510120 广州,中山大学孙逸仙纪念医院泌尿外科2
Author(s):
Xu Hanxin1 Chen Huazhong1 Wu Zhaochun1 Liu Wenbing1 Yang Bangyan1 Huang Hai2.
Department of Urology, Dongguan Qinxi Hospital, Dongguan 523660, China; 2Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
关键词:
间质性膀胱炎抗增殖因子 Occludin Zo-1机制
DOI:
10.3877/cma.j.issn.1674-3253.2015.02.013
摘要:
目的研究紧密连接蛋白 ZO-1(zonula occludens proteins)、Occludin在间质性膀胱炎(IC)与正常对照( NC)之间的表达差异并分析其与抗增殖因子( APF)的关联性,探讨它们在 IC发病中的作用,为阐明 IC的发病机理提供新的思路。方法通过组织块培养法培养正常及 IC膀胱上皮细胞,利用流式细胞检测法测定其纯度;运用离子交换层析等技术纯化 APF,检测 APF对膀胱上皮细胞的抑制效果并评价 APF的活性;以纯化的 APF培养 IC膀胱上皮细胞,以 PBS处理组为对照,采用 Western-blot方法检测 ZO-1及 Occludin在处理前后的表达变化并分析 APF与 Occludin,ZO-1之间的关系。结果组织块培养法成功培养出膀胱上皮细胞,纯化的 APF对膀胱上皮细胞生长的抑制率为 78.5%;IC患者的 ZO-1、Occludin的表达水平均低于正常组,且在经纯化的 APF培养处理的膀胱上皮细胞中, ZO-1、Occludin的表达明显较 PBS对照组低, P<0.01)。结论紧密连接蛋白 ZO-1、Occludin在 IC中呈低表达,纯化的 APF可进一步降低 ZO-1、Occludin的表达水平,提示 APF在 IC中的病因作用可能是通过抑制紧密连接蛋白表达而产生,即 APF可能通过下调紧密连接蛋白 ZO-1、Occludin,破坏膀胱黏膜紧密连接结构,提高膀胱黏膜上皮的通透性,从而促进间质性膀胱炎的发生。

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:广东省自然科学基金资助项目(10151008901000070);国家自然科学基金资助项目(81101947);
通讯作者:黄海,Email:huanghai257@126.com
更新日期/Last Update: 1900-01-01